Immunosuppressant drug extraction reagent for immunoassays

G - Physics – 01 – N

Patent

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Details

G01N 1/34 (2006.01) G01N 1/28 (2006.01) G01N 33/15 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2709272

An improved extractive reagent composition and method for extracting an immunosuppressant drug, such as sirolimus, tacrolimus or cyclosporine, from blood samples while yielding a test sample extract that has low vapor pressure and is compatible with immunoassay components. The inventive reagent composition comprises dimethyl sulfoxide (DMSO), at least one divalent metal salt and water. The sample extracts resulting from use of each of these combinations have low vapor pressure and are compatible with immunochemistry assays.

Cette invention a pour objet une composition de réactif d'extraction améliorée et un procédé pour extraire un immunosuppresseur, tel que le sirolimus, le tacrolimus ou la cyclosporine à partir d'échantillons sanguins qui donne un extrait d'échantillon d'essai qui a une basse pression de vapeur et est compatible avec les composants des dosages immunologiques. La composition de réactif de l'invention comprend du sulfoxyde de diméthyle (DMSO), au moins un sel métallique divalent et de l'eau. Les extraits d'échantillons résultant de l'utilisation de chacune de ces combinaisons ont une basse pression de vapeur et sont compatibles avec les dosages immunochimiques.

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