Immunotoxin containing a disulfide-stabilized antibody fragment

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) C07K 14/21 (2006.01) C07K 16/28 (2006.01) C07K 16/30 (2006.01) C07K 19/00 (2006.01) C12N 15/13 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2234666

This invention provides for immunotoxins comprising a Pseudomonas exotoxin (PE) that does not require proteolytic activation for cytotoxic activity attached to an Fv antibody fragment having a variable heavy chain region bound through at least one disulfide bond to a vairable light chain region. The combination of a "disulfide-stabilized" binding agent fused to a PE that does not require proteolitic activation provides an immunotoxin having surprising cytotoxic activity.

L'invention porte sur des immunotoxines comprenant une exotoxine Pseudomonas (PE), ne nécessitant pas d'activation protéolytique de leur activité cytotoxique et liées à un fragment d'anticorps Fv présentant une région à chaîne lourde variable reliée au moins par l'intermédiaire d'une liaison bisulfure à une région à chaîne légère variable. La combinaison d'un agent de fixation "stabilisé au bisulfure" fusionné avec une PE ne nécessitant pas d'activation protéolytique donne une immunotoxine présentant une activité cytotoxique surprenante.

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