Imoroved stable, single-liquid alph-amylase reagent

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/40 (2006.01)

Patent

CA 2095200

In a single liquid alpha-amylase reagent composition comprising an aqueous solution of at least one substrate which is hydrolyzed when mixed with a sample of body fluid containing alpha-amylase to yield a detectable label, alpha-glucosidase form Bacillus stearothermophilus is used to cooperate with the alpha-amylase in the formation of the detectable label said composition being stable against degradation for a least 6 months at 2 to 10°C.

Dans une composition réactive d'alpha-amylase à un seul liquide comprenant une solution aqueuse d'au moins un substrat qui est hydrolysé lorsqu'il est mélangé à un échantillon de liquide organique contenant de l'alpha-amylase pour produire un marqueur détectable, de l'alpha-glucosidase provenant de Bacillus stearothermophilus est utilisé pour renforcer l'action de l'apha-amylase dans la formation de ladite composition contenant un marqueur détectable, laquelle ne subira pas de dégradation pendant au moins 6 mois, à une température de 2 à 10 degrés Celsius.

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