Improved affinity matrices with enhanced visibility for...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 1/14 (2006.01) B01D 15/00 (2006.01) B01J 20/32 (2006.01) C07K 1/22 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2437381

An affinity matrix for use in affinity based molecular pull down and immunoprecipitation procedures. The affinity matrix comprises a polymeric support, a dye attached to a fraction of the polymeric support to enable optical detection of the polymeric support, and an affinity ligand other than the dye attached to a fraction of the polymeric support for the capture of a molecule. Also provided is a method for the isolation of a biomolecule from an aqueous solution. The method comprises combining the aqueous solution with an affinity matrix comprising a polymeric support and separating the affinity matrix from the aqueous solution. A dye is attached to a fraction of the polymeric support which enables optical detection and monitoring of the affinity matrix and, accordingly, reduces the likelihood of the loss of affinity matrix during the separation step. In addition, an affinity ligand other than the dye is also attached to a fraction of the polymeric support for the capture of the biomolecule.

L'invention concerne une matrice d'affinités s'utilisant dans des procédures d'immunoprécipitation et de capture moléculaire basée sur l'affinité. La matrice d'affinités comprend un support polymère, un colorant lié à une fraction du support polymère permettant la détection optique du support polymère, et un ligand d'affinités autre que le colorant lié à une fraction du support polymère destiné à capturer une molécule. L'invention concerne également un procédé d'isolation d'une biomolécule à partir d'une solution aqueuse. Ce procédé consiste à combiner la solution aqueuse avec une matrice d'affinités comprenant un support polymère et à séparer la matrice d'affinités de la solution aqueuse. Un colorant lié à une fraction du support polymère permet la détection optique et le contrôle de la matrice d'affinités et, réduit en conséquence la vraisemblance de la perte de la matrice d'affinités durant la phase de séparation. En outre, un ligand d'affinités autre que le colorant est également lié à une fraction du support polymère en vue de capturer la biomolécule.

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