Improved automated lpa assay and methods of detecting cancer

Details Improved automated lpa assay and methods of detecting cancer Improved automated lpa assay and methods of detecting cancer

C - Chemistry, Metallurgy

12

Q

C12Q 1/44 (2006.01) C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) C12Q 1/32 (2006.01)

Patent

CA 2389832

The present invention relates to an improved enzymatic diagnostic assay to detect carcinoma by measuring various lysophospholipids, including lysophosphatidic acid (LPA), in a patient. In a preferred embodiment, this assay measures the human plasma level of LPA in an automated format with a minimal number of reagents and with reduced incubation periods. The present invention also comprises several additional technical improvements to the current LPA assays disclosed in the prior art.

La présente invention concerne un test diagnostique enzymatique amélioré permettant de détecter le carcinome grâce à la mesure, chez un patient, de différentes teneurs en lysophospholipides, comprenant l'acide lysophosphatidique (LPA). Dans un mode de réalisation préféré de l'invention, ce test permet de mesurer le taux plasmique humain de LPA sous un format automatisé avec un nombre minimum de réactifs et des temps d'incubation réduits. Cette invention concerne également différentes améliorations techniques supplémentaires apportées aux tests LPA actuels faisant partie de l'état de la technique.

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