Improved barstar gene

Details Improved barstar gene Improved barstar gene

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) A01H 5/00 (2006.01) C07K 14/32 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2234119

Improved barstar DNAs, such as synthetic barstar DNAs, produce high amounts of barstar protein in eucaryotic cells, particularly in plant cells, and especially in stamen cells such as tapetum cells. Synthetic barstar DNAs have less than 40 % A and T nucleotides. Other improved barstar DNAs encode improved barstar proteins which have an N- terminus which starts with Met-Xaa wherein Xaa is Alanine, Valine, Glycine, Aspartic acid or Glutamic acid. Plan cells and plants contain the improved barstar DNAs and/or express the improved barstar protein.

L'invention a pour objet des ADN barstar, tels que des ADN barstar de synthèse, qui produisent de grandes quantités de protéine barstar dans des cellules eucaryotes, en particulier, dans les cellules des plantes, et plus spécialement, dans les cellules d'étamines comme les cellules tapis. Les ADN barstar de synthèse présentent moins de 40 % de nucléotides A et T. D'autres ADN barstar codent des protéines barstar améliorées qui présentent une terminaison N qui commence par Met/Xaa, où Xaa est Alanine, Valine, Glycine, acide aspartique ou acide glutamique. L'invention traite aussi de cellules de plantes et de plantes qui contiennent les ADN barstar améliorés et/ou expriment la protéine barstar améliorée.

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