C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2540310
The present invention relates to an improved method for the bisulfite conversion of DNA. By combination of denaturing solvents, new reaction conditions and new purification methods the efficacy of the bisulfite conversion is clearly improved. The converted DNA can subsequently be analysed by different methods. The present invention facilitates the analysis of cytosine methylation.
La présente invention concerne un procédé amélioré de transformation de bisulfite d'ADN. Par combinaison de solvants dénaturants, de nouvelles conditions de réaction et de nouveaux procédés de purification, l'efficacité de la transformation de bisulfite est clairement améliorée. L'ADN transformé peut ensuite être analysé par différentes techniques. La récente invention facilite l'analyse de la méthylation de la cytosine.
Ballhause Matthias
Berlin Kurt
Cardon Karen
Epigenomics Ag
Macrae & Co.
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