Improved clonal cell lines derived from bti-tn-5b1-4

C - Chemistry – Metallurgy – 12 – N

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C12N 1/00 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2506237

Improved insect cell lines, two of which are designated H5CL-B and H5CL-F (both of which are derived from the parental cell line BTI-TN-5B1-4, ATCC CRL 10859), possess the properties of increased production of baculovirus particles, increased expression of foreign proteins using a baculovirus expression system, and increased resistance to cell culture stress, relative to the parental cell line.

Des lignées cellulaires améliorées d'insecte, dont deux sont dites H5CL-B et H5CL-F (toutes deux dérivées de la lignée cellulaire parentale BTI-TN-5B1-4, ATCC CRL 10859), possèdent des propriétés concernant une production plus élevée de particules de baculovirus, une expression accrue de protéines étrangères au moyen d'un système d'expression du baculovirus, et une résistance plus marquée au stress en culture cellulaire, ceci par rapport à la lignée cellulaire parentale.

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