C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 9/02 (2006.01) C12N 15/64 (2006.01) C12N 15/66 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2411740
The invention concerns a method for producing combinatorial library of functional expression from a combinatorial library of nucleic acids belonging to a common genetic family, comprising a cloning step by recombination in yeast. The invention also concerns a method for producing functional mosaic proteins, and for analysing a functional expression combinatorial library, by determining a sequential imprint for each of the mosaic proteins of the library.
La présente invention concerne un procédé de fabrication de banques combinatoires d'expression fonctionnelle à partir d'une banque combinatoire d'acides nucléiques appartenant à une même famille génique, comprenant une étape de clonage par recombinaison dans la levure. L'invention concerne aussi un procédé de production de protéines mosaïques fonctionnelles, et d'analyse d'une banque combinatoire d'expression fonctionnelle, par détermination d'une empreinte séquentielle pour chacune des protéines mosaïques de la banque.
Abecassis Valerie
Pompon Denis
Truan Gilles
Aventis Pharma S.a.
Centre National de La Recherche Scientifique (cnrs)
Robic
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