Improved compositions and methods for the delivery of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 11/04 (2006.01) A61K 9/00 (2006.01) A61K 9/66 (2006.01) A61K 38/18 (2006.01) A61K 38/33 (2006.01) A61K 48/00 (2006.01) C07K 14/48 (2006.01) C07K 14/70 (2006.01) C12N 15/87 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2169292

This invention provides improved devices and methods for long-term, stable expression of a biologically active molecule using a biocompatible capsule containing genetically engineered cells for the effective delivery of biologically active molecules to effect or enhance a biological function within a mammalian host. The novel capsules of this invention are biocompatible and are easily retrievable. This invention specifically provides improved methods and compositions which utilize cells transfected with recombinant DNA molecules comprising DNA sequences coding for biologically active molecules operatively linked to promoters that are not subject to down regulation in vivo upon implantation into a mammalian host. Furthermore, the methods of this invention allow for the long-term, stable and efficacious delivery of biologically active molecules from living cells to specific sites within a given mammal. In addition, this invention provides a general means for maintaining, for extended periods of time, the in vivo expression of transgenes.

Dispositifs et procédés améliorés pour l'expression stable et prolongée d'une molécule à activité biologique à l'aide d'une capsule biocompatible renfermant des cellules manipulées génétiquement et servant à administrer efficacement des molécules à activité biologique assurant ou améliorant une fonction biologique au sein d'un hôte mammifère. Ces nouvelles capsules sont biocompatibles et récupérables sans difficultés. Plus spécialement, on a prévu des compositions et procédés améliorés mettant en oeuvre des cellules dotées par transfection de molécules d'ADN recombiné comportant des séquences d'ADN qui codent pour des molécules à activité biologique associées activement à des promoteurs non susceptibles de subir une rétrorégulation in vivo lors de leur implantation dans un hôte mammifère. Par ailleurs, ces procédés permettent l'administration stable, efficace et prolongée dans certains sites au sein d'un mammifère déterminé de molécules à activité biologique en provenance de cellules vivantes. On a également prévu un système général d'entretien pendant des durées prolongées de l'expression in vivo de transgènes.

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