Improved compositions for in vitro amplification of nucleic...

Details Improved compositions for in vitro amplification of nucleic... Improved compositions for in vitro amplification of nucleic...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2495051

Method and compositions for improving DNA polymerase and reverse transcriptase reactions are provided. Addition of anti-foam reagents to the reactions improves fluid handling, especially of small volumes and allows enhanced accuracy of optical detection, without substantially inhibiting enzymatic activity.

L'invention concerne des procédés et des compositions destinées à améliorer les réactions faisant appel à une ADN polymérase et à une transcriptase inverse. L'ajout de réactifs antimousse lors des réactions permet d'améliorer la manipulation du fluide, notamment de faibles volumes, et d'accroître la précision de la détection optique, sans inhiber sensiblement l'activité enzymatique.

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