Improved conditionally replicating vectors for inhibiting...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 48/00 (2006.01) A61P 31/12 (2006.01) A61P 31/18 (2006.01) C07K 14/16 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 15/867 (2006.01) C12N 15/90 (2006.01) C12Q 1/04 (2006.01) A61K 35/00 (2006.01)

Patent

CA 2441084

The present invention provides improved conditionally replicating vectors that have improved safety against the generation of replication competent vectors or virus. Also disclosed are methods of making, propagating and selectively packaging, modifying and using vectors. Included are improved helper constructs, host cells, for use with the improved vectors as well as pharmaceutical compositions and host cells comprising the vectors, the use of vector containing host cells to screen drugs, and methods of using the vectors to determine gene function. The methods also include the prophylactic and therapeutic treatment of disease, especially viral infection, and HIV infection in particular.

La présente invention concerne des vecteurs à réplication conditionnelle améliorés assurant une meilleure protection contre la production de vecteurs ou de virus capables de réplication. L'invention concerne également des méthodes de production, de propagation et de conditionnement, de modification et d'utilisation sélectifs de ces vecteurs. L'invention concerne également des gènes hybrides auxiliaires et des cellules hôtes destinés à être utilisés avec lesdits vecteurs améliorés, ainsi que des compositions pharmaceutiques et des cellules hôtes comprenant lesdits vecteurs. L'invention concerne également l'utilisation de vecteurs contenant des cellules hôtes, destinés au criblage de médicaments, ainsi que des méthodes d'utilisation de vecteurs pour déterminer la fonction génique. Ces méthodes sont également destinées au traitement préventif et thérapeutique de pathologies, en particulier d'infections virales, et de l'infection par le VIH en particulier.

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