C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0735 (2010.01) C12N 5/071 (2010.01) C12Q 1/24 (2006.01)
Patent
CA 2675445
While culture medium and systems have been described that permit the culture and proliferation of human embryonic stem cells in feeder free and animal product free conditions, these conditions will not readily support cloning of an embryonic stem cell culture meaning, at least here, the initiation of a sub-culture using one or a very few originating cells. It has been found here that a class of small molecules that are inhibitors of kinase enzymes will increase the efficiency of cloning of stem cell cultures sufficiently to make such cloning practical in the defined medium and in other media as well.
Alors que le milieu et des systèmes de culture ont été décrits permettant la culture et la prolifération de cellules souches embryonnaires humaines dans des conditions exemptes d'alimentation et exemptes de produits d'origine animale, ces conditions ne vont pas nécessairement permettre le clonage d'une culture de cellules souches embryonnaires, c'est-à-dire, au moins dans le cadre présent, l'initiation d'une sous-culture au moyen d'une ou d'un très petit nombre de cellules dd départ. On a observé dans ce cas qu'une classe de petites molécules qui sont inhibitrices d'enzymes kinases vont accroître l'efficacité de clonage de cultures de cellules souches suffisamment pour rendre un tel clonage pratique dans le milieu défini ainsi que dans d'autres milieux.
Bergendahl Veit
Thomson James A.
Gowling Lafleur Henderson Llp
Wisconsin Alumni Research Foundation
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