Improved detection of mage-a expression

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2699853

An oligonucleotide, primer or probe comprises the nucleotide sequence of any of SEQ ID NO. 5, 6, 7, 2, 3, 4, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 25. The oligonucleotides are useful for the detection of the methylation status of a gene, in particular the MAGE-A3 gene. The oligonucleotides are useful in primer pairs, kits and methods for determining the methylation status of the MAGE-A3 gene and for diagnosing cancer, directing therapy and selecting subjects for treatment. The primer or probe can comprise a loop or hairpin structure and can be used in real-time methylation specific PCR.

L'invention concerne un oligonucléotide, une amorce ou une sonde comprenant la séquence nucléotidique d'une quelconque SEQ ID NO. 5, 6, 7, 2, 3, 4, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19 ou 25. Les oligonucléotides sont utilisés dans la détection de l'état de méthylation d'un gène, en particulier le gène MAGE-A3. Les oligonucléotides sont, de plus, utilisés dans des paires d'amorces, des kits et des méthodes de détermination de l'état de méthylation du gène MAGE-A3 et dans le diagnostic du cancer, l'orientation thérapeutique et la sélection de sujets à des fins de traitement. L'amorce ou la sonde peuvent comprendre une structure en boucle ou en épingle à cheveux et peuvent être utilisées dans une PCR spécifique à la méthylation en temps réel.

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