C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/31 (2006.01) C07K 14/24 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 15/67 (2006.01) C12N 15/74 (2006.01) C12P 7/60 (2006.01)
Patent
CA 2294648
Methods are provided for preparing improved fermentation strains of the family Enterobacteriaceae which comprise the steps of eliminating the cryptic plasmid from the progenitor strain thereby creating the improved strain. Methods for reducing the mobilization properties of resident plasmids in an Enterobacteriaceae strain containing a cryptic plasmid are also provided. The present invention provides the nucleic acid sequence of pS, a cryptic plasmid found in Pantoea which can be used to identify the cryptic plasmid in strains of Enterobacteriaceae.
Cette invention se rapporte à des procédés de préparation de souches perfectionnées de fermentation de la famille des Enterobacteriaceae, consistant notamment à éliminer le plasmide cryptique de la souche parente de façon à créer la souche perfectionnée. L'invention concerne également des procédés de réduction des caractéristiques de mobilisation des plasmides résidents dans une souche d'Enterobacteriaceae contenant un plasmide cryptique. Elle se rapporte enfin à la séquence d'acide nucléique de pS, un plasmide cryptique trouvé dans Pantoea qui peut servir à l'identification du plasmide cryptique dans des souches d'Enterobacteriaceae.
Causey Stuart C.
Fowler Timothy
Genencor International Inc.
Smart & Biggar
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