Improved enzymes for the production of 2-keto-l-gulonic acid

Details Improved enzymes for the production of 2-keto-l-gulonic acid Improved enzymes for the production of 2-keto-l-gulonic acid

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12N 15/74 (2006.01) C12P 7/60 (2006.01)

Patent

CA 2144053

Mutants of 2,5-diketo-D-gluconic acid reductase A, an enzyme used to produce 2- keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C) are prepared by site-directed mutagenesis. These mutants have increased catalytic activity, increased expression levels, and/or enhanced temperature stability.

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