Improved expression of hiv polypeptides and production of...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/49 (2006.01) A61K 48/00 (2006.01) C07K 14/16 (2006.01) C07K 14/18 (2006.01) C12N 7/04 (2006.01) C12N 15/867 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2360347

The present invention relates to the efficient expression of HIV polypeptides in a variety of cell types, including, but not limited to, mammalian, insect, and plant cells. Synthetic expression cassettes encoding the HIV Gag-containing polypeptides arc described, as are uses of the expression cassettes in applications including DNA immunization, generation of packaging cell lines, and production of Env-, tat- or Gag-containing proteins. The invention provides methods of producing Vitus-Like Particles (VLPs), as well as, uses of the VLPs including, but not limited to, vehicles for tire presentation of antigens and stimulation of immune response in subjects to whom the VLPs are administered.

Cette invention concerne l'expression efficace de polypeptides HIV dans divers types de cellules, y compris, mais pas exclusivement dans des cellules de mammifères, d'insectes et de végétaux. Sont décrites des cassettes d'expression synthétique qui codent pour des polypeptides renfermant HIB-Gag ainsi que l'utilisation desdites cassettes d'expression dans des applications telles que l'immunisation par l'ADN, la production de lignées cellulaires d'encapsidation, et la production de protéines renfermant Env-, tat- ou Gag. L'invention porte sur des méthodes permettant de produire des particules de tape viral (VLP),ainsi que sur des méthodes d'utilisation de ces particules comprenant notamment, mais pas exclusivement, des vecteurs pour la présentation d'antigènes de VLP et la simulation de réponses immunitaire chez des sujets auxquels des VLP ont été administrées.

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