Improved expression of influenza a m2 protein in baculovirus...

Details Improved expression of influenza a m2 protein in baculovirus... Improved expression of influenza a m2 protein in baculovirus...

C - Chemistry, Metallurgy

12

N

C12N 15/44 (2006.01) C07K 14/11 (2006.01) C12N 5/02 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2111116

2111116 9303173 PCTABS00019 The present invention relates to baculovirus-expressed influenza antigens, in particular, to the influenza A membrane protein, M2, expressed from Autographa Californica nuclear polyhedrosis virus (AcNPV). The present invention further relates to a method to increase the yield of baculovirus-expressed M2 proteins in host cells by culturing the recombinant baculovirus infected host cells with an amantadine-like drug. Other aspects of the present invention relate to the use of baculovirus-expressed M2 proteins in reproducible and routine assays for the seradiagnosis of influenza A virus infections as an alternative to the more burdensome complement fixation and hemagglutination tests.

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