Improved expression vectors

Details Improved expression vectors Improved expression vectors

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01) C12N 15/74 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2271230

The present invention provides isolated recombinant nucleic acid constructs comprising a dual bacterial promoter operably linked to a heterologous nucleic acid which encodes a desired polypeptide. The constructs are useful for the expression of the desired polypeptides in bacterial cells at high levels.

La présente invention concerne des acides nucléiques de recombinaison isolés comprenant un double promoteur bactérien fonctionnellement lié à un acide nucléique hétérologue qui code un polypeptide désiré. Ces acides nucléiques de recombinaison peuvent être utilisés pour l'expression des polypeptides désirés dans des cellules bactériennes, à des taux élevés.

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