Improved extraction process

C - Chemistry – Metallurgy – 07 – B

Patent

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C07B 63/00 (2006.01) C07K 2/00 (2006.01)

Patent

CA 2518722

A process for isolating low molecular weight (~LMW~) peptides from tissue in situ comprising the steps: a) homogenizing the tissue; b) mixing the homogenized tissue with an organic solvent to form a fully-wetted slurry; c) standing or agitating the slurry to denature the proteins in situ within the tissue; d) removing the organic solvent from the tissue; e) mixing the organic solvent-treated tissue of step d) with a sufficient volume of water or an aqueous solution to extract the peptides; f) separating a liquid extract from the tissue residue of step e) to obtain an aqueous solution containing the low molecular weight peptide fraction removed from the tissue.

L'invention concerne un processus permettant d'isoler des peptides à faible poids moléculaire ( LMW ) à partir de tissu in situ, lequel processus comprend les étapes consistant: a) à homogénéiser le tissu, b) à mélanger le tissu homogénéisé avec un solvant organique pour former une pâte complètement mouillée, c) à immobiliser ou agiter la pâte pour dénaturer les protéines in situ dans le tissu, d) à éliminer le solvant organique du tissu, e) à mélanger le tissu traité avec le solvant organique de l'étape d) avec un volume d'eau suffisant ou une solution aqueuse pour extraire les peptides, f) à séparer un extrait liquide du résidu de tissu de l'étape e) pour obtenir une solution aqueuse contenant la fraction de peptide à faible poids moléculaire éliminée du tissu.

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