Improved flow cytometer nozzle and flow cytometer sample...

Details Improved flow cytometer nozzle and flow cytometer sample... Improved flow cytometer nozzle and flow cytometer sample...

G - Physics

01

N

G01N 15/10 (2006.01) C12Q 1/04 (2006.01) C12N 5/071 (2010.01) C12N 5/076 (2010.01) A61B 17/425 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2739572

A method of flow cytometry sample processing, comprising the steps of establishing a sheath fluid; injecting a sample into said sheath fluid at an injection point; subjecting said sample to a first axial motion surface in a nozzle; transitioning to a second axial motion surface in said nozzle; subjecting said sample to said second axial motion surface in said nozzle wherein said first and said second axial motion surfaces transition with a maximal acceleration differentiation; coordinating said maximal acceleration differentiation so as to not exceed the practical capabilities of said sample over its length; affirmatively limiting said maximal acceleration differentiation so as to not exceed the practical capabilities of said sample over its length; exiting said sample from said nozzle; analyzing said sample.

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