Improved fret-probes and use thereof

G - Physics – 01 – N

Patent

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Details

G01N 33/542 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2706477

This invention relates to the detection of among others tumor-specific fusion proteins and protein interactions. Provided is a set of at least a first and a second molecular probe, each probe provided with a dye wherein said dyes together allow energy transfer, each probe additionally provided with a reactive group allowing juxtaposing said at least first and second probe, wherein said reactive group is an oligonucleotide and wherein the reactive group of said first probe is not directly reactive with the reactive group of said second probe.

La présente invention concerne la détection, entre autres, de protéines de fusion spécifiques à une tumeur et d'interactions protéiques. L'invention concerne, plus précisément, un ensemble d'au moins une première et une deuxième sonde moléculaires, chaque sonde étant pourvue d'un colorant et lesdits colorants permettant conjointement le transfert d'énergie. Chaque sonde est, en outre, dotée d'un groupe réactif permettant la juxtaposition desdites première et deuxième sondes, ledit groupe réactif étant un oligonucléotide et le groupe réactif de ladite première sonde n'étant pas directement réactif avec le groupe réactif de ladite deuxième sonde.

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