Improved gene expression

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/85 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2443275

Polynucleotides and vectors comprising an expressible nucleic acid flanked by a 5' extended methylation-free CpG island and a 3' selectable marker element are disclosed. Such polynucleotides and vectors provide a means for obtaining high levels of expression of the flanked expressible nucleic acid. Preferred embodiments include combinations of 5' extended methylation-free CpG islands and 3' antibiotic resistance genes.

La présente invention se rapporte à des polynucléotides et à des vecteurs comportant un acide nucléique susceptible d'être exprimé et flanqué d'un îlot CpG sans méthylation et à extension en 5' ainsi qu'un élément marqueur susceptible d'être sélectionné en 3'. Ces polynucléotides et vecteurs fournissent un moyen d'obtention de niveaux élevés d'expression de l'acide nucléique susceptible d'être exprimé et flanqué. Les modes de réalisation préférés incluent des combinaisons d'îlots CpG sans méthylation et à extension en 5' et de gènes dotés d'une résistance aux antibiotiques en 3'.

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