Improved gene silencing methods

Details Improved gene silencing methods Improved gene silencing methods

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2695455

Provided are methods and means to obtain improved gene silencing of target nucleic acids whereby at least two inhibitory RNA molecules are provided which are targeted to the same nucleic acid, but which are processed into short interfering RNA molecules through different processing pathways. Also provided are methods and means to obtain improved gene silencing of target nucleic acids whereby at least two inhibitory RNA molecules are provided which are targeted to different nucleic acids, but which are processed into short interfering RNA molecules through different processing pathways.

L'invention porte sur des procédés et des moyens pour obtenir un silençage génique amélioré d'acides nucléiques cibles. Les procédés et les moyens fournissent au moins deux molécules d'ARN inhibitrices ciblées sur le même acide nucléique mais traitées en courtes molécules d'ARN interférentes par différentes voies de traitement. L'invention porte également sur des procédés et des moyens pour obtenir un silençage génique amélioré d'acides nucléiques cibles. Les procédées et les moyens fournissent au moins deux molécules d'ARN inhibitrices ciblées sur différents acides nucléiques mais traités en courtes molécules d'ARN interférentes par différentes voies de traitement.

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