Improved genetic elements providing high levels of expression

Details Improved genetic elements providing high levels of expression Improved genetic elements providing high levels of expression

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2514412

The invention relates to improved genetic elements providing high levels of expression of operably-linked genes in a variety of tissues. In particular, fragments of unmethylated, CpG islands of less than 2kb are shown to provide enhanced transgene expression and have advantages in terms of vector construction and cloning capacity.

La présente invention se rapporte à des éléments génétiques améliorés apportant des taux élevés d'expression de gènes liés de manière fonctionnelle dans une grande variété de tissus. Plus particulièrement, des fragments d'îlots CpG non méthylés de moins de 2 kb se sont révélés fournir une expression transgénique améliorée et présenter des avantages en termes de construction de vecteur et de capacité de clonage.

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