C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/04 (2006.01) C12N 5/10 (2006.01) C12N 15/52 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2535147
The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a plynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comrpising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.
La présente invention porte sur des polypeptides de glucose déshydrogénase (GDH) dont l'activité et/ou la thermostabilité par rapport au polypeptide de glucose déshydrogénase de type sauvage, à squelette, sont accrues. L'invention porte également sur un polynucléotide qui code pour les polypeptides de GDH de l'invention, sur des séquences d'acide nucléique comprenant les polynucléotides, sur des vecteurs d'expression comprenant les polynucléotides liés de manière fonctionnelle à un promoteur, sur des cellules hôtes transformées pour exprimer les polypeptides de GDH et sur un procédé de production de ces polypeptides de GDH.
Davis S. Christopher
Jenne Stephane J.
Krebber Anke
Newman Lisa Marie
Codexis Inc.
Smart & Biggar
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