Improved immortalized human skin cell lines and novel...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/10 (2006.01) A61K 35/36 (2006.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01) C12Q 1/02 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2234118

The present invention relates to improved continuous (immortalized) cell lines, in particular keratinocytes and melanocytes derived from normal human skin tissue. The present invention also relates to novel serum-free media for isolating, producing and maintaining said improved continuous keratinocyte and melanocyte cell lines. The present invention also relates to methods for producing primary melanocytes and keratinocytes under serum-free conditions without any feeder cells.

La présente invention concerne une amélioration apportée à des lignées cellulaires continues (immortalisées), en particulier des kératinocytes et des mélanocytes dérivés de tissus normaux de la peau humaine. La présente invention concerne également de nouvelles substances sans sérum servant à isoler, produire et maintenir lesdites lignées cellulaires continues, améliorées, de kératinocytes et de mélanocytes. La présente invention concerne en outre des procédés pour produire des kératinocytes et des mélanocytes primaires dans des conditions sans sérum et sans avoir recours à des cellules nourricières

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