Improved immunoassay including lyophilized reactant mixture

Details Improved immunoassay including lyophilized reactant mixture Improved immunoassay including lyophilized reactant mixture

G - Physics

01

N

G01N 33/535 (2006.01) C12N 9/96 (2006.01) G01N 33/543 (2006.01) G01N 33/546 (2006.01) G01N 33/548 (2006.01) G01N 33/553 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2053885

IMPROVED IMMUNOASSAY INCLUDING LYOPHILIZED REACTANT MIXTURE Abstract A lypphilized mixture of reactants for an immunoassay includes antibody-gold sol particle conjugates, antibody latex par- ticle conjugates, polyethylene glycol p-isooctylphenyl ether detergent and dextrin or trehalose. The polyeth- ylene glycol is present to enhance binding of the immunoreactants and the polyethylene glycol p-isooctylphenyl ether detergent is present to prevent non-specific interactions. The dextrin or threhalose prevents agglomeration of the polyethylent glycol and pol- yethylene galycol p-isooctylphenyl ether in the lyophilized mixture at room temperature and facilitates retention of homogenous distribution of the ingredients of the mixture to thereby enhance shelf life and redistribution of the mixture in an aqueous test sys- tem.

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