C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/50 (2006.01) C12N 9/96 (2006.01) C12Q 1/28 (2006.01) C12Q 1/68 (2006.01) G01N 1/28 (2006.01) G01N 1/30 (2006.01) G01N 33/535 (2006.01) G01N 33/543 (2006.01) G01N 33/567 (2006.01) G01N 33/58 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2077451
The present invention provides an improved method for staining slides using immunochemical reagents. The method com- prises the following steps. The assay region of a slide (the region containing the tissue section) is washed with an improved rin- sing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent contain- ing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. Color development reagents, including, in a preferred em- bodiment, a stabilized diaminobenzidine (DAB) solution, are combined with the tissue section for a time sufficient for color de- velopment. Following rinsing, the tissue section is ready for analysis.
Degroff Michael J.
Gizinski Michael J.
Hartman Anthony L.
Miller Phillip C.
Rybski James A.
Dennison Associates
Ventana Medical Systems Inc.
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