Improved isothermal strand displacement amplification

Details Improved isothermal strand displacement amplification Improved isothermal strand displacement amplification

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2749939

A method for isothermal DNA amplification comprising: providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing Xanthosine, a second primer at least partially complementary to a region of DNA and containing Xanthosine, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises Xanthosine in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near Xanthosine; and amplifying the DNA substantially without thermal cycling.

Méthode d'amplification d'ADN isotherme comprenant : le fait d'apporter à l'ADN à amplifier un mélange d'amplification comprenant une première amorce au moins partiellement complémentaire d'une région de l'ADN et contenant de la xanthosine, une seconde amorce au moins partiellement complémentaire d'une région de l'ADN et contenant de la xanthosine, une ADN polymérase, une enzyme pouvant déplacer des brins, une enzyme reconnaissant la xanthosine dans l'ADN double brin et provoquant une encoche ou excisant une base dans un brin d'ADN au niveau de la xanthosine ou à proximité ; et l'amplification de l'ADN sans cyclage thermique substantiel.

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