Improved ketoreductase polypeptides and related polynucleotides

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/04 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2533838

The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention.

La présente invention se rapporte à des variantes de polypeptides possédant une activité cétoréductase et/ou une thermostabilité améliorées, destinés à servir à la réduction stéréospécifique des cétones. De plus, l'invention a trait à des polynucléotides codant lesdits polypeptides cétoréductase, notamment des versions optimisées par codons des polynucléotides, qui permettent d'améliorer l'expression dans des cellules hôtes. Dans un autre aspect, la présente invention concerne des nucléotides de synthèse, des vecteurs et des cellules hôtes qui sont transformés grâce aux polynucléotides selon l'invention.

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