Improved method for bisulfite treatment

Details Improved method for bisulfite treatment Improved method for bisulfite treatment

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2438327

The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non- methylated cytosines, whereby the nucleic acid is bound to a solid phase during the deamination and/ or desulfonation step of the bisulfite reaction. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, the use of a solid phase for binding a nucleic acid during the deamination and/ or desulfonation step of the bisulfite reaction is disclosed and a kit containing a bisulfite reagent and a solid phase.

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