C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01)
Patent
CA 2547743
The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses uses of guanidinium hydrogen sulfilte and kits containing it.
L'invention a trait à la détection d'une cytosine méthylée dans un acide nucléique, du sulfite d'hydrogène de guanidinium étant employé pour préparer une solution renfermant des ions guanidinium et des ions sulfite ; et à la modification ultérieure de l'acide nucléique. Le procédé comprend la transformation d'une cytosine non méthylée en uracile. L'invention concerne de plus l'utilisation de sulfite d'hydrogène de guanidinium et des trousses contenant celui-ci.
Birkner Christian
Markert-Hahn Christine
von Der Eltz Herbert
F. Hoffmann-La Roche Ag
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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