C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/31 (2006.01) C12Q 1/04 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2447750
A microorganism causative of an infection is quickly and highly sensitively detected and/or identified by obtaining phagocytes from a clinical specimen containing phagocytes originating in a living body, immobilizing the obtained phagocytes, treating the phagocytes so as to enhance the permeability of the phagocyte membrane and expose DNA of the microorganism causative of the infection which is anticipated as being contained in the phagocytes, and then using a detection DNA probe hybridizable with the DNA under stringent conditions.
L'invention porte sur un procédé permettant de détecter et d'identifier rapidement et avec une grande sensibilité un micro-organisme cause d'infection consistant: à prélever sur un échantillon clinique des phagocytes provenant d'un corps vivant; à les immobiliser; à les traiter de manière à accroître la porosité de leur membrane et à exposer l'ADN du micro-organisme cause d'infection supposé être présent dans les phagocytes; et à utiliser une sonde d'ADN hybridable avec l'ADN dans des conditions strictes.
Matsuhisa Akio
Ohno Tsuneya
Fuso Pharmaceutical Industries Ltd.
Kirby Eades Gale Baker
Ohno Tsuneya
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