C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12Q 1/68 (2006.01) G01N 1/34 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01)
Patent
CA 2144328
The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies direct-ed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotin--complexes or certain enzymes allowing visualization, will dramatically increase the specificity of the method. The method can fur-ther be used for isolation of the target-cells by magnetic field application and a kit for performing the method according to the invention is described.
Fodstad Oystein
Kvalheim Gunnar
Fodstad Oystein
Goudreau Gage Dubuc
Kvalheim Gunnar
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