Improved method for isolating and recovering target dna or...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2294465

The invention generally concerns the use of amino acid denaturants for denaturing or separating double standed nucleic acid molecules. More specifically, the present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of haptenylated probes and amino acid denaturants to select the desired molecules and eliminate the undesired library members from a sample. The invention also provides a method in which larger or full-length nucleic acid molecules can be isolated from the subpopulation of desired molecules.

De manière générale, l'invention concerne l'utilisation de dénaturants d'acides aminés destinés à dénaturer ou séparer des molécules d'acides nucléiques bicaténaires. Plus particulièrement, la présente invention se rapporte à un procédé permettant d'isoler et récupérer rapidement des molécules cibles souhaitées d'ADN ou ARN dans un mélange ou une banque contenant ces molécules. Ce procédé consiste à utiliser des sondes hapténylées et des dénaturants d'acides aminés pour choisir les molécules souhaitées et éliminer les éléments de la banque indésirables dans un échantillon. Cette invention concerne également un procédé selon lequel des molécules d'acides nucléiques plus longues ou entières peuvent être isolées au sein de la sous-population de molécules désirées.

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