Improved method for more efficient electroporation

Details Improved method for more efficient electroporation Improved method for more efficient electroporation

C - Chemistry, Metallurgy

12

N

C12N 15/64 (2006.01) C12N 13/00 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2345352

This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.

Cette invention porte sur des procédés améliorés d'électroporation permettant de transférer des acides nucléiques dans des cellules hôtes. Les cellules hôtes sont (1) en suspension dans une solution pratiquement non ionique comprenant au moins un sucre ou un dérivé du sucre, (2) mélangées aux acides nucléiques, et (3) traitées électriquement. Cette invention porte également sur des kits utilisés dans ce procédé visant à transférer des acides nucléiques dans des cellules hôtes.

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