Improved method for purifying cholera toxin

Details Improved method for purifying cholera toxin Improved method for purifying cholera toxin

G - Physics

01

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G01N 33/53 (2006.01) A61K 39/106 (2006.01) A61K 39/108 (2006.01) C07K 14/245 (2006.01) C07K 14/28 (2006.01) G01N 33/569 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2322846

The invention relates to a method of purifying cholera toxin or the enterotoxin of E. coli using a matrix with at least on e ion chosen from among matrix with Ni+2, Co+2, Cd2 or Zn+2 immobilized thereon. It is possible thereby to selectively elute the B subunit for cholera toxin from the matrix.

Cette invention se rapporte à un procédé pour purifier la toxine cholérique ou l'entérotoxine de E. coli, en utilisant une matrice ayant au moins un ion Ni?+2¿, Co?+2¿, Cd?2¿ ou Zn?+2¿ immobilisé sur ladite matrice. Il est ainsi possible de séparer sélectivement par élution la sous-unité B de toxine cholérique de ladite matrice.

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