Improved method for the detection of creatinine

Details Improved method for the detection of creatinine Improved method for the detection of creatinine

G - Physics

01

N

G01N 33/70 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2281166

Disclosed is an improvement to the assay for creatinine in fluid test samples by contacting the test sample with a reagent formulation containing cupric ions, a hydroperoxide and an oxidizable dye. The improvement involves the inclusion of one or more selected quinolines in the reagent formulation. When the quinoline is substituted with methyl in the 2-5 position and/or a methoxy in the 2-6 position auto-oxidation of the oxidizable dye is reduced. When the quinoline is further substituted in an otherwise unsubstituted carbon atom in the 2-8 position, the assay's resistance to interference from hemoglobin and ascorbate is increased.

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