G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2311896
The present invention relates to an improved method for the identification and optionally the characterisation of interacting molecules designed to perform two-hybrid screening in a high throughput fashion. The method of the invention relies on a combination of automated steps used to generate and detect clones that express interacting molecules, and to separate positive from false positive clones. The present invention provides for high-throughput interaction screens for the reliable identification of interacting molecules, which in turn can lead to the identification of substances inhibiting said interactions. Such inhibitors can find their use in the formulation of a pharmaceutical composition.
L'invention porte sur une technique améliorée d'identification et facultativement de caractérisation de molécules interagissantes permettant d'effectuer des criblages de 2H avec un fort rendement, ladite technique reposant sur une combinaison d'étapes automatisées permettant de produire et de détecter des clones exprimant des molécules interagissantes, et de séparer les clones positifs des clones faussement positifs. L'invention assure des criblages à fort rendement permettant d'identifier fiablement des molécules interagissantes qui, à leur tour, peuvent conduire à l'identification de substances inhibant lesdites interactions, et pouvant entrer dans la composition de préparations pharmaceutiques.
Bancroft David
Lehrach Hans
Maier Elmar
Meier-Ewert Sebastian
Wanker Erich
Borden Ladner Gervais Llp
Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.v.
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