Improved method for the refolding of proteins

Details Improved method for the refolding of proteins Improved method for the refolding of proteins

C - Chemistry, Metallurgy

07

K

C07K 1/113 (2006.01) C07D 413/14 (2006.01) C07D 471/04 (2006.01) C07K 1/00 (2006.01) C07K 1/14 (2006.01) C07K 14/47 (2006.01) C07K 14/61 (2006.01) C07K 14/705 (2006.01) C07K 14/81 (2006.01) C12N 9/00 (2006.01) C12N 9/64 (2006.01) C12N 9/68 (2006.01)

Patent

CA 2155335

A novel, generally applicable method for producing correctly folded proteins from a mixture of misfolded proteins, e.g. bacterial inclusion-body aggregates. A major new aspect of the method is that over-all efficiency is achieved by subjecting proteins to a time- sequence of multiple denaturation-renaturation cycles, resulting in gradual accumulation of the correctly folded protein. The method has proven efficient for a variety of recombinant proteins. Also provided are novel encrypted recognition sites for bovine coagulation factor Xa. The encrypted recognition sites described may be activated in vitro by controlled oxidation or by reversible derivatization of cysteine residues and thereby generate new cleavage sites for factor Xa. Two new recombinant serine protease exhibiting narrow substrate specificity for factor Xa recognition sites are also provided. They may replace natural coagulation factor Xa for cleavage of chimeric proteins.

Affiliated with

Also associated with

Rating

Improved method for the refolding of proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Improved method for the refolding of proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Improved method for the refolding of proteins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1824274