Improved method of expression cloning in a host cell

Details Improved method of expression cloning in a host cell Improved method of expression cloning in a host cell

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01)

Patent

CA 2599640

DSM IP Assets B.V. 24403WO IMPROVED METHOD OF EXPRESSION CLONING IN A HOST CELL 5 ABSTRACT The invention relates to a promoter DNA sequence highly suited in an improved expression cloning method for isolation of DNA sequences comprising a DNA sequence encoding a protein of interest in a host cell and to the improved expression cloning method wherein use is made of this promoter. The isolated DNA sequences are useful in processes for 10 producing a protein of interest.

Cette invention concerne une séquence d'ADN promoteur parfaitement appropriée pour être utilisée dans un procédé de clonage par expression amélioré destiné à isoler des séquences d'ADN contenant une séquence d'ADN codant une protéine recherchée dans une cellule hôte, ainsi qu'un procédé de clonage par expression amélioré, dans lequel on utilise ce promoteur. Ces séquences d'ADN isolées sont utiles dans des procédés de production d'une protéine recherchée.

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