C - Chemistry – Metallurgy – 40 – B
Patent
C - Chemistry, Metallurgy
40
B
C40B 30/04 (2006.01) C12Q 1/68 (2006.01) C40B 80/00 (2006.01) C40B 40/02 (2006.01) C40B 50/06 (2006.01) C40B 70/00 (2006.01)
Patent
CA 2737035
The present invention features improved methods of in vitro RNA display to allow reliable expression and selection of scFv antibody molecules from expression libraries. The improved methods, in part, involve the use of mildly reducing conditions, which favor of scFv intra-chain disulphide bond and thus correct folding of the scFv antibody molecules. Although particularly suited to expression and selection of scFv antibody molecules, the methods of the invention are also expedient for in vitro RNA display of all classes of protein.
La présente invention concerne des procédés améliorés daffichage dARN in vitro pour permettre de manière fiable lexpression et la sélection de molécules danticorps scFv provenant de banques dexpression. Les procédés améliorés impliquent, en partie, lutilisation de conditions réductrices douces qui favorisent la formation dun pont disulfure à lintérieur de la chaîne du scFv et qui corrigent donc le repliement des molécules danticorps scFv. Bien que les procédés de linvention soient particulièrement appropriés à lexpression et à la sélection de molécules danticorps scFv, ils peuvent également être utilisés pour laffichage dARN in vitro de toutes les classes de protéines.
Hsieh Chung-Ming
Kutskova Yuliya A.
Memmott John E.
Abbott Laboratories
Torys Llp
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