Improved methods and materials for transformation

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01H 3/00 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2329509

Disclosed herein are novel methods and materials directed to transforming a host cell and expressing exogenous RNA therein. Specifically disclosed are DNA-launching platforms used to introduce a replicating viral segment attached to an exogenous polynucleotide into a cell, whereby the exogenous polynucleotide is expressed in said cell and confers a detectable trait.

L'invention concerne de nouvelles méthodes et de nouveaux matériels destinés à la transformation d'une cellule hôte et à l'expression dans celle-ci d'ARN exogène. Spécifiquement, l'invention concerne des plates-formes de lancement d'ADN utilisées pour introduire un segment viral réplicant fixé à un polynucléotide exogène dans une cellule, de manière que le polynucléotide exogène soit exprimé dans ladite cellule et confère un trait détectable.

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