Improved methods and means for producings hyaluronan

Details Improved methods and means for producings hyaluronan Improved methods and means for producings hyaluronan

A - Human Necessities

01

H

A01H 5/00 (2006.01) A01H 5/08 (2006.01) C12N 5/14 (2006.01) C12N 9/04 (2006.01) C12N 9/06 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2624973

The present invention relates to plant cells and plants which synthesize an increased amount of hyaluronan, and to methods for preparing such plants, and also to methods for preparing hyaluronan with the aid of these plant cells or plants. Here, plant cells or genetically modified plants according to the invention have hyaluronan synthase activity and additionally an increased glutamine:fructose 6-phosphate amidotransferase (GFAT) activity and an increased UDP glucose dehydrogenase (UDP-GIc-DH) activity, compared to wild- type plant cells or wild-type plants. The present invention furthermore relates to the use of plants having increased hyaluronan synthesis for preparing hyaluronan and food or feedstuff containing hyaluronan.

La présente invention concerne des cellules végétales et des végétaux qui synthétisent une quantité accrue de hyaluronan et des procédés de préparation de ces végétaux, ainsi que des procédés de préparations d'hyaluronan à l'aide de ces cellules végétales ou de ces végétaux. Des cellules végétales ou des végétaux génétiquement modifiés de l'invention possèdent une activité de hyaluronan synthase et par ailleurs une activité glutamine:fructose 6-phosphate amidotransferase (GFAT) accrue ainsi qu'une activité UDP glucose déshydrogenase (UDP-GIc-DH) accrue, comparées à celle des cellules végétales de type sauvage ou des végétaux de type sauvage. Cette invention concerne aussi l'utilisation de végétaux possédant une synthèse de l'hyaluronan accrue afin de préparer de l'hyaluronan et des aliments contenant de l'hyaluronan.

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