Improved methods controlling gene expression

Details Improved methods controlling gene expression Improved methods controlling gene expression

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 15/79 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2604807

The present invention is in the field of genetics, especially plant genetics, and provides agents capable of controlling gene expression. The present invention specifically pro vides sequences of naturally occurring, tissue- specifically expressed microRNAs. The invention further provides for transgenic expression constructs comprising sequences encoding said microRNAs. By incorporation of the microRNA encoding sequence the expression from said expression construct is specifically silenced in the tissue where the naturally occurring microRNA is naturally expressed. Thereby the expression profile resulting from the promoter is modulated and leakiness is reduced. The invention fur ther provides for a method for modulating transgenic expression by incorporating se quences encoding said microRNAs into transgenic expression constructs. The compositions and methods of the invention can be used to enhance performance of agricul tural relevant crops and for therapy, prophylaxis, research and diagnostics in diseases and disorders, which afflict mammalian species.

L'invention concerne le domaine de la génétique, en particulier la génétique végétale. L'invention concerne des agents permettant de contrôler une expression génique. L'invention concerne en particulier des séquences de microARN exprimées spécifiquement dans des tissus d'origine naturel. L'invention concerne également des constructions d'expression transgénique comprenant des séquences codant lesdits microARN. L'incorporation de la séquence codant le microARN permet d'effectuer un silençage spécifique de l'expression de la construction d'expression dans le tissu dans lequel le microARN d'origine naturelle est naturellement exprimé. Le profil d'expression obtenu à partir du promoteur est modulé et les fuites sont réduites. L'invention concerne également une méthode pour moduler une expression transgénique par l'incorporation de séquences codant les microARN susmentionnés pour obtenir des constructions d'expression transgénique. Les compositions et les méthodes de l'invention peuvent être utilisées pour accroître la performance de cultures agricoles pertinentes et pour une thérapie, une prophylaxie, la recherche et le diagnostic de maladies et de troubles touchant des espèces de mammifère.

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