Improved methods for detecting and measuring specific...

C - Chemistry – Metallurgy – 40 – B

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C40B 30/10 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01) C40B 40/06 (2006.01) G01N 33/52 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2545252

The invention provides novel oligonucleotides and methods of using the same for detection or measurement of specific nucleic acid molecules. The invention also features nucleic acid arrays comprising the oligonucleotides of the invention. An oligonucleotide of the invention comprises (1) a reporter- binding sequence capable of hybridizing to a fluorrophore-labeled reporter sequence and (2) a hairpin-forming sequence capable of forming a stem-loop. Formation of the stem-loop modifies (e.g., quenching) the fluorescence signals of the reporter sequence when the reporter sequence is hybridized to the oligonucleotide. This can be achieved, for example, by bringing one or more guanine based in the oligonucleotide into close proximity to the fluorophore(s) of the reporter sequence by virtue of the formation of the stem- loop. Disruption of the stem-loop, such as by hybridization of a target sequence to at least part of the hairpin-forming sequence, produces a detectable change in the fluorescence signals.

La présente invention a trait à de nouveaux oligonucléotides et leurs procédés d'utilisation pour la détection et la mesure de molécules d'acides nucléiques spécifiques. L'invention a également trait à des jeux ordonnés d'échantillons d'acides nucléiques comportant les oligonucléotides de l'invention. Un oligonucléotide de l'invention comporte (1) une séquence de liaison à un gène rapporteur capable d'hybridation à une séquence rapporteuse marquée au fluorophore et (2) une séquence de formation d'épingle à cheveux capable d'une formation tige-boucle. La formation tige-boucle modifie (par exemple, une extinction) des signaux de fluorescence de la séquence rapporteuse lors le l'hybridation de la séquence rapporteuse à l'oligonucléotide. Cela peut se réaliser, par exemple, par le rapprochement d'une ou de plusieurs guanines basées dans l'oligonucléotide au(x) fluorophore(s) de la séquence rapporteuse grâce à la formation tige-boucle. La dissociation de la tige-boucle, telle que par l'hybridation d'une séquence cible à au moins une partie de la séquence formant épingle à cheveux, produit une modification détectable dans les signaux de fluorescence.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Improved methods for detecting and measuring specific... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Improved methods for detecting and measuring specific..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Improved methods for detecting and measuring specific... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-2015685

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.