Improved methods for determining binding affinities

Details Improved methods for determining binding affinities Improved methods for determining binding affinities

G - Physics

01

N

G01N 33/557 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2458795

The present invention relates generally to methods for screening a plurality of ligands using a biosensor device. More particularly, the present invention relates to methods for screening a plurality of antibodies from complex solutions using a surface plasmon resonance device. The methods of this invention provide kinetic and equilibrium information for such screening assays. The present invention also relates to systems for determining kinetic rate constants for such screening assays.

La présente invention se rapporte de manière générale à des procédés de criblage d'une pluralité de ligands utilisant un dispositif biocapteur. L'invention se rapporte plus particulièrement à des procédés de criblage d'une pluralité d'anticorps à partir de solutions complexes utilisant un dispositif de résonance de placement de surface. Les procédés de cette invention fournissent une information cinétique et d'équilibre pour de tels tests de criblage. La présente invention se rapporte également à des systèmes de détermination de constantes de vitesse cinétique pour de tels tests de criblage.

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