C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07H 1/06 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2270023
The present invention provides rapid and highly effective methods for extracting nucleic acids suitable for PCR amplification from tissue samples and paraffin-embedded tissue samples. Extraction is accomplished within a few minutes using a composition comprising a buffer, at least one nonionic surfactant, and a protease enzyme. The sample is then heated at alkaline pH and after a centrifugation step, the DNA is the supernatant can be used directly in known amplification methods, such as PCR.
La présente invention traite de méthodes rapides et très efficaces permettant d'extraire des acides nucléiques destinés à être amplifiés par PCR à partir d'échantillons de tissus et d'échantillons de tissus en bloc de paraffine. L'extraction est réalisée en quelques minutes à l'aide d'une composition comprenant un tampon, au moins un surfactant non ionique et une protéase. L'échantillon est alors chauffé à pH alcalin et, après une étape de centrifugation, l'ADN du surnageant peut être utilisé directement dans des méthodes d'amplification connues, comme le PCR.
Belly Robert T.
Chilson Gary J.
Gowling Lafleur Henderson Llp
Ortho-Clinical Diagnostics Inc.
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