Improved methods for storing neural cells

Details Improved methods for storing neural cells Improved methods for storing neural cells

A - Human Necessities

01

N

A01N 1/02 (2006.01) A01N 63/00 (2006.01) A61K 35/30 (2006.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01) A01N 65/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2333303

The instant methods pertain to improved methods for storing neural cells, preferably dissociated neural cells, prior to their use in transplantation and to the cells obtained using such methods. One embodiment pertains to methods for storing the neural cells in medium lacking added buffer or added protein, other embodiments feature neural cells which are maintained at 4 ~C prior to cryopreservation and have comparable viability and/or functionality to freshly harvested cells. In addition, methods for storing and/or transplantation of porcine neural cells are described.

L'invention concerne des procédés améliorés qui permettent de stocker des cellules neuronales, de préférence des cellules neuronales dissociées, avant leur utilisation aux fins de transplantation. L'invention concerne également des cellules résultant de la mise en oeuvre desdits procédés. Selon une variante, les procédés décrits consistent à stocker les cellules neuronales dans un milieu sans tampon ajouté ni protéine ajoutée et, selon d'autres variantes, les cellules neuronales sont maintenues à une température de 4· C avant la cryoconservation, ayant par ailleurs une viabilité et/ou une fonctionnalité que l'on peut comparer à la viabilité et/ou à la fonctionnalité de cellules fraîchement recueillies. L'invention concerne en outre des procédés relatifs au stockage et/ou à la transplantation de cellules neuronales porcines.

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